Pengaruh Ekstraksi Bertingkat Terhadap Kadar Flavonoid Total Ekstrak Binahong (Anredera cordifolia (Ten) Steenis)
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http://orcid.org/0000-0002-3946-8407
Arief Kusuma Wardani
Ellsya Angeline Rawar
Abstract
Binahong ( Anredera cordifolia (Ten.) Steenis) merupakan tumbuhan yang memiliki banyak khasiat dan sering digunakan untuk mengobati berbagai macam penyakit. Salah satu kandungan senyawa yang terdapat dalam daun binahong adalah flavonoid yang berfungsi sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstraksi maserasi bertingkat terhadap kadar total flavonoid ekstrak binahong. Metode yang digunakan dalam penelitian ini adalah eksperimental. Daun binahong di ekstraksi secara maserasi bertingkat dengan menggunakan pelarut yang memiliki kepolaran berbeda. Pelarut yang digunakan terdiri atas pelarut n-heksana (nonpolar), etil asetat (semi polar), dan etanol 96% (polar). Analisis kualitatif pada penelitian ini menggunakan uji tabung dan analisis kuantitatif menggunakan metode spektrofotometri UV-Vis pada panjang gelombang maksimum 417,5 nm dengan pembanding standar rutin. Hasil analisis kualitatif ekstrak n-heksan, ekstrak etil asetat, dan ekstrak etanol 96% mengandung flavonoid positif. Berdasarkan pengujian kuantitatif diperoleh kadar flavonoid total ekstrak n-heksan, etil asetat, dan etanol 96% berturut-turut yakni sebesar 2,3102%; 3,6124%; dan 1,0264%. Hal ini menunjukkan bahwa penggunaan ekstraksi maserasi bertingkat memberikan pengaruh terhadap kadar total flavonoid yang dihasilkan pada masing-masing ekstrak.
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